Research data is the original Intellectual Property produced by researcher that is then reported and interpreted in scholarly books, journal articles, and conference proceedings. This collection is a store of research data produced by Oxford Brookes researchers so that their data can be accessed by other researchers and the public.
This essay was first published in the Europe Japan Research Centre Occassional Paper series in 1998 as a revised and extended version of a paper presented for the EJRC 15th June 1998. For a preliminary report in Japanese see Kuwayama 1996.
The sections in this papers include
I. Personal Backrgound
II. Japan's Place in American Anthropology
III. A Content Analysis of American Textbooks of Anthropology
IV. Some Theoretical Issues
V. Concluding Remarks
Originally published as part of the Europe Japan Research Centre Occasional Paper series (3/3) in September 2007.
Includes an overview of games and gambling in Japan with particular attention to Pachinko, racing (horse racing, cycle racing, boat racing), Mahjong, and Casinos.
As a teaching resource for the INDEPTH Academy Frederic Pontvianne introduces the Functions of the Plant Nucleolus
In this teaching webinar for the INDEPTH Academy Dr Monica Pradillo Introduces the Role of Chromatin Domains on Plant Meiosis.
This article describes a protocol for Fluorescence-Activated
Nucleolar Sorting (FANoS), which allows for clean extraction of the nucleolus from a range of plant tissues. This generates a nucleolar extract that is appropriate for use for downstream multi-‘omic characterisation of this important nuclear sub-domain.
Video interview with Maria Quinto (Bianco) as part of the Italian Cinema Audiences project
Analysis of individual cell types can add significant understanding to our knowledge the processes that drive the formation of complex tissues. However one major challenge in this area involves the difficulty in separating cells with different identifies that may be buried deep within tissues. Recently cell-type specific expression of fluorescent reporter proteins has facilitated the isolation of different cell types; initially by isolation of protoplasts and now through the use of flow cytometry and cell sorting. This latter technique allows the more rapid separation of the nuclei from specific cell files to ensure that a more realistic in-planta situation is revealed upon subsequent downstream analysis. This article provides a consensus methodology for the isolation of labelled nuclei and for the processing of these samples for analysis of gene expression, methylation state and of chromatin interactions.
Visualising the location of the total cellular mRNA pool can be important to understanding how different genes effect cellular physiology. Over the past decade researchers investigating RNA processing, nuclear transport and the function of the nuclear pore complex have used this situ hybridization protocol to visualize and quantify the accumulation of the total mRNA pool within the plant cell nucleus.
Traditional in situ hybridization is a powerful tool for the localization of RNA molecules within plant tissues. However the sensitivity of this technique is limited to single cell resolution and is unable to discriminate between sub-cellular expression domains. Single molecule fluorescent in situ hybridization (smFISH) is an evolution of this technique that allows for the resolution of expression to the level of a single messenger or non-coding RNA. This technique relies on the use of multiple fluorescent probes targeted to a single RNA sequence, which amplifies the signal such that is it visible using epi-fluorescent imaging. This allows both the localization and the quantification of single RNA molecules thus providing an increased level of precision in the study of gene expression.
The nuclear space is a dynamic environment in which DNA molecules interact across time, space and scale. Within the nucleus different yet adjacent chromosomes are co- regulated through shared chromatin modifications or the influence of global enhancer or repressor units. The interactions between adjacent chromosomes can be analysed though Hi-C, a technique that takes a whole genome view on samples obtained by chromosome confirmation capture. This technique has been used in many different plant species and this article provides a consensus protocol that is merged from those used in two expert labs.
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