Journal Article

CEP164C regulates flagellum length in stable flagella


Cilia and flagella are required for cell motility and sensing the external environment and can vary in both length and stability. Stable flagella maintain their length without shortening and lengthening and are proposed to “lock” at the end of growth, but molecular mechanisms for this lock are unknown. We show that CEP164C contributes to the locking mechanism at the base of the flagellum in Trypanosoma brucei . CEP164C localizes to mature basal bodies of fully assembled old flagella, but not to growing new flagella, and basal bodies only acquire CEP164C in the third cell cycle after initial assembly. Depletion of CEP164C leads to dysregulation of flagellum growth, with continued growth of the old flagellum, consistent with defects in a flagellum locking mechanism. Inhibiting cytokinesis results in CEP164C acquisition on the new flagellum once it reaches the old flagellum length. These results provide the first insight into the molecular mechanisms regulating flagella growth in cells that must maintain existing flagella while growing new flagella.

Attached files


Atkins, Madison
Týč, Jiří
Shafiq, Shahaan
Ahmed, Manu
Bertiaux, Eloïse
De Castro Neto, Artur Leonel
Sunter, Jack D.
Bastin, Philippe
Dean, Samuel Dale
Vaughan, Susan

Oxford Brookes departments

Department of Biological and Medical Sciences


Year of publication: 2020
Date of RADAR deposit: 2020-11-26

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License

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