Journal Article


Lysophosphatidic acid acyltransferases: A link with intracellular protein trafficking in Arabidopsis root cells?

Abstract

Phosphatidic acid (PA) and Lysophosphatidic acid acyltransferases (LPAATs) might be critical for the secretory pathway. Four extra-plastidial LPAATs (LPAAT2, 3, 4 and 5) were identified in A. thaliana. These AtLPAATs, display a specific enzymatic activity converting lysophosphatidic acid (LPA) to PA and are located in the endomembrane system. We investigate a putative role of the AtLPAATs 3, 4 and 5 in the secretory pathway of root cells through genetical (knock-out mutants), biochemical (activity inhibitor, lipid analyses) and imaging (live and immuno-confocal microscopy) approaches. Treating a lpaat4;lpaat5 double mutant with the LPAAT inhibitor CI976 showed a significant decrease in primary root growth. The trafficking of the auxin transporter PIN2 was disturbed in this lpaat4;lpaat5 double mutant treated with CI976, whereas trafficking of H+-ATPases was unaffected. The lpaat4;lpaat5 double mutant is sensitive to salt stress and the trafficking of the aquaporin PIP2;7 to the plasma membrane in the lpaat4;lpaat5 double mutant treated with CI976 was reduced. We measured the amounts of neo-synthesized PA in roots, and found a decrease in PA only in the lpaat4;lpaat5 double mutant treated with CI976, suggesting that the protein trafficking impairment was due to a critical PA concentration threshold.



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Embargo end: 2022-11-19

Authors

Wattelet-Boyer, Valérie
Le Guédard, Marina
Dittrich-Domergue, Franziska
Maneta-Peyret, Lilly
Kriechbaumer, Verena
Boutté, Yohann
Bessoule, Jean-Jacques
Moreau, Patrick

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year of publication: 2021
Date of RADAR deposit: 2021-11-10



Related resources

This RADAR resource is the Accepted Manuscript of Lysophosphatidic acid acyltransferases: A link with intracellular protein trafficking in Arabidopsis root cells?
This RADAR resource is the Accepted Manuscript of [bioRxiv preprint] Lyso-Phosphatidic Acid Acyl-Transferases: A link with intracellular protein transport in Arabidopsis root cells?

Details

  • Owner: Joseph Ripp
  • Collection: Outputs
  • Version: 1 (show all)
  • Status: Live