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Hi-C protocol for analysis of plant nuclear chromatin interactions

Abstract

The nuclear space is a dynamic environment in which DNA molecules interact across time, space and scale. Within the nucleus different yet adjacent chromosomes are co- regulated through shared chromatin modifications or the influence of global enhancer or repressor units. The interactions between adjacent chromosomes can be analysed though Hi-C, a technique that takes a whole genome view on samples obtained by chromosome confirmation capture. This technique has been used in many different plant species and this article provides a consensus protocol that is merged from those used in two expert labs.

Subjects

INDEPTH_3,

DOI (Digital Object Identifier)

Permanent link to this resource: https://doi.org/10.24384/39fw-jm66

Attachments

Authors

Parry, Geraint

Contributors

Project Members: Liu, Chang

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year: 2020

Funding

European Cooperation in Science and Technology (COST) : Impact of Nuclear Domains On Gene Expression and Plant Traits (CA16212)


Published by Oxford Brookes University

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License


Related resources

This RADAR resource is Part of INDEPTH COST Action CA16212

Details

  • Owner: Geraint Parry
  • Collection: Research
  • Version: 1 (show all)
  • Status: Live
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