Book Chapter


Two-photon imaging

Abstract

This chapter will provide an overview of two-photon microscopy from elements of the theory underpinning fluorescence phenomena to functioning principles of a two-photon microscope including step-by-step practical advice on how to conduct an experiment using a two-photon microscope. In this context multi-photon excitation is also taken into consideration. By reading this chapter, you will have a synopsis of the basic principles of two-photon excitation, optical sectioning, and 3D microscopy. Furthermore, fundamentals of promising advanced methods for tissue imaging available for two-photon imaging as second harmonic generation (SHG) and fluorescence lifetime imaging microscopy (FLIM) are briefly described together with classical applications on deep tissue imaging and functional brain imaging.

Attached files

Authors

Sancataldo, Giuseppe
Barrera, Olga
Vetri, Valeria

Oxford Brookes departments

School of Engineering, Computing and Mathematics

Dates

Year of publication: 2022
Date of RADAR deposit: 2023-03-13



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Related resources

This RADAR resource is the Accepted Manuscript of Two-Photon Imaging
This RADAR resource is Part of Principles of light microscopy: From basic to advanced

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