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Isolation of the Plant Nucleolus

Abstract

This article describes a protocol for Fluorescence-Activated Nucleolar Sorting (FANoS), which allows for clean extraction of the nucleolus from a range of plant tissues. This generates a nucleolar extract that is appropriate for use for downstream multi-‘omic characterisation of this important nuclear sub-domain.

DOI (Digital Object Identifier)

Permanent link to this resource: https://doi.org/10.24384/ycxk-1685

Attachments

Authors

Parry, Geraint

Contributors

Project Members: Pontvianne, Frederic

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year: 2020

Funding

European Cooperation in Science and Technology (COST) : Impact of Nuclear Domains On Gene Expression and Plant Traits (CA16212)

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License

Details

  • Owner: Geraint Parry
  • Collection: Research
  • Version: 1 (show all)
  • Status: Live
  • Views (since Sept 2022): 61