Journal Article


Control of assembly of extra-axonemal structures: The paraflagellar rod of trypanosomes

Abstract

Eukaryotic flagella are complex microtubule based organelles and in many organisms there are extra axonemal structures present, including the outer dense fibres of mammalian sperm and the paraflagellar rod (PFR) of trypanosomes. Flagellum assembly is a complex process occurring across three main compartments, the cytoplasm, the transition fibre-transition zone, and the flagellum. It begins with translation of protein components, followed by their sorting and trafficking into the flagellum, transport to the assembly site and then incorporation. Flagella are formed from over 500 proteins; the principles governing axonemal component assembly are relatively clear. However, the coordination and sites of extra-axonemal structure assembly processes are less clear. We have discovered two cytoplasmic proteins in T. brucei that are required for PFR formation, PFR assembly factors 1 and 2. Deletion of either PFR-AF1 or PFR-AF2 dramatically disrupted PFR formation and caused a reduction in the amount of major PFR proteins. The presence of cytoplasmic factors required for PFR formation aligns with the concept of processes occurring across multiple compartments to facilitate axoneme assembly and this is likely a common theme for extra-axonemal structure assembly.

Attached files

Authors

Alves, Aline A.
Gabriel, Heloisa B.
Bezerra, Maria J.R.
de Souza, Wanderley
Vaughan, Sue
Cunha-e-Silva, Narcisa L.
Sunter, Jack D.

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year of publication: 2020
Date of RADAR deposit: 2020-04-01


Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License


Related resources

This RADAR resource is the Accepted Manuscript of Control of assembly of extra-axonemal structures: The paraflagellar rod of trypanosomes
This RADAR resource is the Accepted Manuscript of Control of assembly of extra-axonemal structures: The paraflagellar rod of trypanosomes [preprint]

Details

  • Owner: Joseph Ripp
  • Collection: Outputs
  • Version: 1 (show all)
  • Status: Live