Journal Article

Serial block face scanning electron microscopy and the reconstruction of plant cell membrane systems

Abstract

Serial block face imaging with the scanning electron microscope has been developed as an alternative to serial sectioning and transmission electron microscopy for the ultrastructural analysis of the three dimensional organisation of cells and tissues. An ultramicrotome within the microscope specimen chamber, permits sectioning and imaging to a depth of many microns within resin embedded specimens. The technology has only recently been adopted by plant microscopists and here we describe some specimen preparation procedures suitable for plant tissue, suggested microscope imaging parameters and discuss the software required for image reconstruction and analysis.

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Authors

Hawes, Chris
Kittelmann, Maike
Hughes, Louise

Oxford Brookes departments

Faculty of Health and Life Sciences\Department of Biological and Medical Sciences

Dates

Year of publication: 2016
Date of RADAR deposit: 2016-06-13

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

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Related resources

This RADAR resource is the Accepted Manuscript of Serial block face scanning electron microscopy and the reconstruction of plant cell membrane systems