Book Chapter

Overexpression of Endoplasmic Reticulum (ER) proteins from Arabidopsis thaliana in baculovirus

Abstract

The overproduction of proteins of the Endoplasmic Reticulum (ER) of plant cells in prokaryotic heterologue gene expression system remains a technical challenge. Recent advances in genetically modified insect cells technology and virus engineering methods have paved the way to produce recombinant ER plant proteins, including those harbouring post-translational modifications and therefore, to yield ER plant proteins that are natively folded and fully functional. The present contribution focuses on the baculovirus-expression system flashBac, which overcomes certain technical hurdles found in other insect cells-based expression systems such as the generation of a bacmid and the negative selection of recombinant clones.

Authors

Bolanos-Garcia, Victor M.

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year of publication: 2024
Date of RADAR deposit: 2024-03-13

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Related resources

This RADAR resource is the Accepted Manuscript of Overexpression of Endoplasmic Reticulum (ER) proteins from Arabidopsis thaliana in baculovirus
This RADAR resource is Part of The plant endoplasmic reticulum: methods and protocols [ISBN: 9781071637098] / edited by Verena Kriechbaumer (Springer, 2024).