Journal Article


The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis

Abstract

Plant plasma-membrane (PM) proteins are involved in several vital processes, such as detection of pathogens, solute transport and cellular signalling. For these proteins to function effectively there needs to be structure within the PM allowing, for example, proteins in the same signalling cascade to be spatially organized. Here we demonstrate that several proteins with divergent functions are located in clusters of differing size in the membrane using sub-diffraction-limited Airyscan confocal microscopy. Single particle tracking reveals that these proteins move at different rates within the membrane. Actin and microtubule cytoskeletons appear to significantly regulate the mobility of one of these proteins (the pathogen receptor FLS2) and we further demonstrate that the cell wall is critical for the regulation of cluster size by quantifying single particle dynamics of proteins with key roles in morphogenesis (PIN3) and pathogen perception (FLS2). We propose a model in which the cell wall and cytoskeleton are pivotal for regulation of protein cluster size and dynamics thereby contributing to the formation and functionality of membrane nanodomains.

Attached files

Authors

McKenna, Joseph F.
Rolfe, Daniel J.
Webb, Stephen E.D.
Tolmie, Andrea F.
Botchway, Stanley W.
Martin-Fernandez, Marisa L.
Hawes, Christopher
Runions, John

Oxford Brookes departments

Faculty of Health and Life Sciences\Department of Biological and Medical Sciences

Dates

Year of publication: 2019
Date of RADAR deposit: 2019-06-06


Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License


Related resources

This RADAR resource is the Version of Record of The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis
This RADAR resource is the Version of Record of The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis

Details

  • Owner: Joseph Ripp
  • Collection: Outputs
  • Version: 1 (show all)
  • Status: Live