Journal Article


Model based biotechnological potential analysis of Kluyveromyces marxianus central metabolism

Abstract

The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modeling and optimisation methods. Our model reveals the organism's potential for industrial application and metabolic engineering. Modeling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.

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Authors

Pentjuss, A
Stalidzans, E
Liepins, J
Kokina, A
Martynova, J
Zikmanis, P
Mozga, I
Scherbaka, R
Hartman, H
Poolman, M
Fell, D
Vigants, A

Oxford Brookes departments

Faculty of Health and Life Sciences\Department of Biological and Medical Sciences

Dates

Year of publication: 2017
Date of RADAR deposit: 2017-04-27


Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License


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