Journal Article

The odd one out: Arabidopsis reticulon 20 does not bend ER membranes but has a role in lipid regulation

Abstract

Reticulons are integral ER membrane proteins characterised by a reticulon homology domain comprising four transmembrane domains which results in the proteins sitting in the membrane in a W-topology. Here we report on a novel subgroup of reticulons with an extended N-terminal domain and in particular on arabidopsis reticulon 20. Using high resolution confocal microscopy we show that reticulon 20 is located in a unique punctate pattern on the ER membrane. Its closest homologue reticulon 19 labels the whole ER. Other than demonstrated for the other members of the reticulon protein family RTN20 and 19 do not display ER constriction phenotypes on over expression. We show that mutants in RTN20 or RTN19, respectively, display a significant change in sterol composition in roots indicating a role in lipid regulation. A third homologue in this family -3BETAHSD/D1- is unexpectedly localised to ER exit sites resulting in an intriguing location difference for the three proteins.

Attached files

Authors

Kriechbaumer, Verena
Maneta-Peyret, Lilly
Fouillen, Laetitia
Botchway, Stanley W.
Upson, Jessica
Hughes, Louise
Richardson, Jake
Kittelmann, Maike
Moreau, Patrick
Hawes, Chris

Oxford Brookes departments

Faculty of Health and Life Sciences\Department of Biological and Medical Sciences

Dates

Year of publication: 2018
Date of RADAR deposit: 2018-01-25

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This work is licensed under a Creative Commons Attribution 4.0 International License

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