Fluralaner is a novel insecticide targeting the ionotropic GABA receptor (GABAR) subunit, RDL. A recent study revealed that N316L, a substitution of asparagine (N) with leucine (L), in the second transmembrane (M2)-spanning region reduced the antagonist action of fluralaner on the housefly Musca domestica RDL (MdRDL) in vitro. To verify the impact of N316L in vivo, the corresponding mutation (N318L) in the fruitfly Drosophila melanogaster RDL (DmRDL) was constructed using CRISPR/Cas9 genome editing. The homozygous DmRDLN318L mutant showed a 9.87-fold resistance to fluralaner compared with w1118 while still being highly sensitive to broflanilide and fipronil, which is consistent with those findings observed in the electrophysiology assays of the homomeric DmRDLWT or DmRDLN318L channel. Moreover, DmRDLN318L led to malformed ovaries, stunted eggs, and sterility in homozygous females. These results highlighted N318 as a molecular site for fluralaner in vivo and in vitro and might elucidate the resistance mechanisms of insects against fluralaner.
The fulltext files of this resource are currently embargoed.Embargo end: 2025-10-01
Zhang YichiLiu XinyuWang JunyanWang YingAmponsah, PriscillaTang TaoJones, Andrew J. Zhao Chunqing
School of Biological and Medical Sciences
Year of publication: 2024Date of RADAR deposit: 2024-10-08
“This document is the unedited author’s version of a work accepted for publication in Journal of Agricultural and Food Chemistry, copyright © 2024 after peer review. To access the final edited and published work see https://doi.org/10.1021/acs.jafc.4c06478."