Journal Article

Genome-wide subcellular protein localisation in the flagellate parasite Trypanosoma brucei


Trypanosoma brucei is a model trypanosomatid, an important group of human, animal and plant unicellular parasites. Understanding their complex cell architecture and life cycle is challenging because, as with most eukaryotic microbes, ~50% of genome-encoded proteins have completely unknown functions. Here, using fluorescence microscopy and cell lines expressing endogenously tagged proteins, we mapped the subcellular localization of 89% of the T. brucei proteome, a resource we call TrypTag. We provide clues to function and define lineage-specific organelle adaptations for parasitism, mapping the ultraconserved cellular architecture of eukaryotes, including the first comprehensive ‘cartographic’ analysis of the eukaryotic flagellum, which is vital for morphogenesis and pathology. To demonstrate the power of this resource, we identify novel organelle subdomains and changes in molecular composition through the cell cycle. TrypTag is a transformative resource, important for hypothesis generation for both eukaryotic evolutionary molecular cell biology and fundamental parasite cell biology.

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Billington, Karen
Halliday, Clare
Madden, Ross
Dyer, Philip
Carrington, Mark
Vaughan, Sue
Hertz-Fowler, Christiane
Dean, Samuel
Sunter, Jack D.
Wheeler, Richard John
Gull, Keith

Oxford Brookes departments

Department of Biological and Medical Sciences


Year of publication: 2023
Date of RADAR deposit: 2022-11-29

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License

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