Journal Article


Expression of clementine asp-rich proteins (CcASP-RICH) in tobacco plants interferes with the mechanism of pollen tube growth

Abstract

Low-molecular-weight, aspartic-acid-rich proteins (ASP-RICH) have been assumed to be involved in the self-incompatibility process of clementine. The role of ASP-RICH is not known, but hypothetically they could sequester calcium ions (Ca2+) and affect Ca2+-dependent mechanisms. In this article, we analyzed the effects induced by clementine ASP-RICH proteins (CcASP-RICH) when expressed in the tobacco heterologous system, focusing on the male gametophyte. The aim was to gain insight into the mechanism of action of ASP-RICH in a well-known cellular system, i.e., the pollen tube. Pollen tubes of tobacco transgenic lines expressing CcASP-RICH were analyzed for Ca2+ distribution, ROS, proton gradient, as well as cytoskeleton and cell wall. CcASP-RICH modulated Ca2+ content and consequently affected cytoskeleton organization and the deposition of cell wall components. In turn, this affected the growth pattern of pollen tubes. Although the expression of CcASP-RICH did not exert a remarkable effect on the growth rate of pollen tubes, effects at the level of growth pattern suggest that the expression of ASP-RICH may exert a regulatory action on the mechanism of plant cell growth.

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Authors

Parrotta, Luigi
Mareri, Lavinia
Aloisi, Iris
Faleri, Claudia
Distefano, Gaetano
Gentile, Alessandra
Lo Piero, Angela Roberta
Kriechbaumer, Verena
Caruso, Marco
Cai, Giampiero
Del Duca, Stefano

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year of publication: 2022
Date of RADAR deposit: 2022-07-26


Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License


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