Traditional in situ hybridization is a powerful tool for the localization of RNA molecules within plant tissues. However the sensitivity of this technique is limited to single cell resolution and is unable to discriminate between sub-cellular expression domains. Single molecule fluorescent in situ hybridization (smFISH) is an evolution of this technique that allows for the resolution of expression to the level of a single messenger or non-coding RNA. This technique relies on the use of multiple fluorescent probes targeted to a single RNA sequence, which amplifies the signal such that is it visible using epi-fluorescent imaging. This allows both the localization and the quantification of single RNA molecules thus providing an increased level of precision in the study of gene expression.
INDEPTH_4,
Permanent link to this resource: https://doi.org/10.24384/4h9v-ar37
Parry, Geraint
Project Members: Rosa, Stefanie
Department of Biological and Medical Sciences
Year: 2020
European Cooperation in Science and Technology (COST) : Impact of Nuclear Domains On Gene Expression and Plant Traits (CA16212)