Book Chapter


Preparation of ER microsomes from Arabidopsis thaliana

Abstract

Microsomes are vesicles derived from the endoplasmic reticulum (ER) when cells are broken down in the lab. These microsomes are a valuable tool to study a variety of ER functions such as protein and lipid synthesis in vitro. Here we describe a protocol to isolate ER-derived microsomes Arabidopsis thaliana seedlings and exemplify the use of these purified microsomes in enzyme assays with the auxin precursors tryptophan (Trp) or indole-3-pyruvic acid (IPyA) to quantify auxin synthetic capacity in microsomal and cytosolic fractions.



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Authors

Kriechbaumer, Verena

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year of publication: 2024
Date of RADAR deposit: 2024-03-06


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Related resources

This RADAR resource is the Accepted Manuscript of Preparation of ER Microsomes from Arabidopsis thaliana
This RADAR resource is Part of 2024.03.06: Springer allows one chapter per contributor: allocate this to Spatola Rossi (Brookes postgThe plant endoplasmic reticulum: methods and protocols [ISBN: 9781071637098] / edited by Verena Kriechbaumer (Springer, 2024).

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