Book Chapter

Preparation of ER microsomes from Arabidopsis thaliana

Abstract

Microsomes are vesicles derived from the endoplasmic reticulum (ER) when cells are broken down in the lab. These microsomes are a valuable tool to study a variety of ER functions such as protein and lipid synthesis in vitro. Here we describe a protocol to isolate ER-derived microsomes Arabidopsis thaliana seedlings and exemplify the use of these purified microsomes in enzyme assays with the auxin precursors tryptophan (Trp) or indole-3-pyruvic acid (IPyA) to quantify auxin synthetic capacity in microsomal and cytosolic fractions.

Attached files

Authors

Kriechbaumer, Verena

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year of publication: 2024
Date of RADAR deposit: 2024-03-06

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Related resources

This RADAR resource is the Accepted Manuscript of Preparation of ER Microsomes from Arabidopsis thaliana
This RADAR resource is Part of 2024.03.06: Springer allows one chapter per contributor: allocate this to Spatola Rossi (Brookes postgThe plant endoplasmic reticulum: methods and protocols [ISBN: 9781071637098] / edited by Verena Kriechbaumer (Springer, 2024).