Analysis of individual cell types can add significant understanding to our knowledge the processes that drive the formation of complex tissues. However one major challenge in this area involves the difficulty in separating cells with different identifies that may be buried deep within tissues. Recently cell-type specific expression of fluorescent reporter proteins has facilitated the isolation of different cell types; initially by isolation of protoplasts and now through the use of flow cytometry and cell sorting. This latter technique allows the more rapid separation of the nuclei from specific cell files to ensure that a more realistic in-planta situation is revealed upon subsequent downstream analysis. This article provides a consensus methodology for the isolation of labelled nuclei and for the processing of these samples for analysis of gene expression, methylation state and of chromatin interactions.
Permanent link to this resource: https://doi.org/10.24384/fvmf-km57
Parry, Geraint
Project Members: Guztat, Ruben
Department of Biological and Medical Sciences
Year: 2020
European Cooperation in Science and Technology (COST) : Impact of Nuclear Domains On Gene Expression and Plant Traits (CA16212)