Journal Article


Broflanilide prolongs the development of fall armyworm Spodoptera frugiperda by regulating biosynthesis of juvenile hormone

Abstract

Broflanilide, a novel meta-diamide insecticide, has been registered worldwide to control agricultural pests, and cause negative influence to insect under not only lethal but also sublethal level in the field. Fall armyworm (FAW) Spodoptera frugiperda is a worldwide distributed insect frequently controlled by using insecticides, and the potential regulating mechanism of broflanilide on this key insect prompt careful characterization. In the present study, we exposed FAW larvae to sublethal dose (LD5), low lethal doses (LD10 and LD30) and median lethal dose (LD50) of broflanilide and measured various subsequent physiological sublethal effects. FAW larvae body length became shorter (LD5-LD50), the larvae and pupae duration were increased by 0.96-4.63 days (LD5-LD50), and the juvenile hormone (JH) titer significantly increased up to 134.46% (LD10-LD30). Meanwhile, the JH acid methyltransferase gene (JHAMT) and farnesyl diphosphate synthase 1 (FPPS1), which are critical enzymes of JH biosynthesis, increased by 2.07- and 2.18- fold in LD10 broflanilide-treated group, and by 2.22- and 1.78- fold in LD30 broflanilide-treated group in 3rd day larvae of FAW, respectively. In the 12-hour-old adults, SfrFPPS1 increased to 1.92-fold in LD30 broflanilide-treated group. Broflanilide induced multiple physiological sublethal effects on the biosynthesis of JH by regulating the expression of SfrFPPS and SfrJHAMT genes in FAW and likely in other insects (both pests and non-target organisms). Therefore, its potential for Integrated Pest Management should be further assessed.

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Authors

Jia Zhong Qiang
Zhan En Ling
Zhang Su Gui
Wang Ying
Song Ping Ping
Jones, Andrew K.
Han Zhao Jun
Zhao Chun Qing

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year of publication: 2022
Date of RADAR deposit: 2022-05-23


Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License


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