Journal Article


Malaria and Dengue mosquito vectors from Lao PDR show a lack of the rdl mutant allele responsible for cyclodiene insecticide resistance

Abstract

The gamma-aminobutyric acid (GABA) receptor, RDL, plays important roles in neuronal signalling and is the target of highly effective insecticides. A mutation in RDL, commonly A296S, underlies resistance to several insecticides such as cyclodienes. Even though the use of cyclodienes has been banned, the occurrence of mutations substituting A296 is notably high in mosquitoes from several countries. Here we report a survey investigating the prevalence of the Rdl mutant allele in mosquitoes from Laos, a country where mosquito-borne diseases such as malaria and dengue fever are health concerns. Anopheles and Aedes mosquitoes were collected from twelve provinces in Laos. Adult bioassays on Ae. aegypti (Linnaeus) (Diptera: Culicidae) and Ae. albopictus (Skuse) showed that all the populations tested were susceptible to dieldrin (4%) following WHO protocols. Exon 7 from a total of 791 mosquitoes was sequenced to identify the amino acid encoded for at 296 of RDL. Only one of these mosquitoes, Anopheles maculatus rampae (Diptera: Culicidae) from Attapeu, carried the mutant allele being heterozygous for A296S. We therefore found a general lack of the Rdl mutant allele indicating that mosquitoes from Laos are not exposed to insecticides that act on the GABA receptor compared to mosquitoes in several other countries. Identifying the prevalence of the Rdl mutation may help inform the potential use of alternative insecticides that act on the GABA receptor should there be a need to replace pyrethroids in order to prevent/manage resistance.

Attached files

Authors

Marcombe, Sebastien
Thammavong, Phoutmany
Luangamath, Phonesavanh
Chonephetsarath, Somsanith
Phommavanh, Nothasin
Lakeomany, Khaitong
Nilaxay, Somphat
Rahmani, Zuhal
Saverton, Penelope
Abdullateef, Omobolanle H.
Forward, Jordan
Jacob, Anna E.
Khadam, Safina
Ali, Wlaa
Boer, Chloé
Kakinuma, Hayato
Hawkins, Joseph
Longstreeth, Rosie
Portwood, Natalie M.
Smee, Madeleine
Brown, Natasha
Kuyucu, Nursu C.
Lechmere, Susannah
Stieger, Gabriela
Maithaviphet, Santi
Nambanya, Simone
Brey, Paul T.
Jones, Andrew K.

Oxford Brookes departments

Department of Biological and Medical Sciences

Dates

Year of publication: 2019
Date of RADAR deposit: 2019-11-14



“This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Medical Entomology following peer review. The version of record is available online at: https://doi.org/10.1093/jme/tjz227.”


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