Journal Article


Protein storage vacuoles originate by remodelling of pre-existing vacuoles in Arabidopsis thaliana

Abstract

Protein storage vacuoles (PSV) are the main repository of protein in dicotyledonous seeds. Little is known about the origins of these transient organelles. PSV are hypothesised to either arise de novo or to originate from the pre-existing embryonic vacuole (EV) during seed maturation. We have tested these hypotheses by studying PSV formation in Arabidopsis embryos at different stages of seed maturation and have recapitulated this process in Arabidopsis leaves reprogrammed to an embryogenic fate by inducing expression of the LEAFY COTYLEDON2 transcription factor. Confocal and immunoelectron microscopy indicate that both storage proteins and tonoplast proteins typical of PSV are delivered to the pre-existing EV in embryos or to the lytic vacuole in reprogrammed leaf cells. In addition, sectioning through embryos at several developmental stages using serial block face scanning electron microscopy reveals the 3D architecture of forming PSV. Our results indicate that in Arabidopsis the pre-existing vacuole is reprogrammed to become a PSV.

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Authors

Feeney, Mistianne
Kittelmann, Maike
Menassa, Rima
Hawes, Chris
Frigerio, Lorenzo

Oxford Brookes departments

Faculty of Health and Life Sciences\Department of Biological and Medical Sciences

Dates

Year of publication: 2018
Date of RADAR deposit: 2018-03-16


Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License


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This RADAR resource is the Accepted Manuscript of Protein storage vacuoles originate by remodelling of pre-existing vacuoles in Arabidopsis thaliana

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