Journal Article


The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology

Abstract

Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila.

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Authors

Schmitt-Engel, C
Schultheis, D
Schwirz, J
Ströhlein, N
Troelenberg, N
Majumdar, U
Anh Dao, V
Grossmann, D
Ritcher, T
Tech, M
Dönitz, J
Gerischer, L
Theis, M
Schild, I
Trauner, J
Koniszewski, N
Küster, E
Kittelmann, S
Hu, Y
Lehmann, S
Siemanowski, J
Ulrich, J
Panfilio, KA
Schröder, R
Morgenstern, B
Stanke, M
Buchhholz, F
Frasch, M
Roth, S
Wimmer, E
Schoppmeier, M
Kingler, M
Bucher, G

Oxford Brookes departments

Faculty of Health and Life Sciences

Dates

Year of publication: 2015
Date of RADAR deposit: 2017-05-23


Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License


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