Flatworms of the species Schmidtea mediterranea are immortal –adult animals contain a large pool of pluripotent stem cells that continuously differentiate to all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. Here, by combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from the transcriptional changes, and a novel lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterize gene expression changes during differentiation and discover cell types important for regeneration. Our results demonstrate
the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at unprecedented resolution.
Plass, MireyaSolana, JordiWolf , F. AlexanderAyoub, SalahMisios, AristotelisGlažar, PetarObermayer, BenediktTheis, Fabian J.Kocks, ChristineRajewsky, Nikolaus
Faculty of Health and Life Sciences\Department of Biological and Medical Sciences
Year of publication: 2018Date of RADAR deposit: 2018-07-06