Journal Article


Cell type atlas and lineage tree reconstruction of whole adult animals by single-cell transcriptomics

Abstract

Flatworms of the species Schmidtea mediterranea are immortal –adult animals contain a large pool of pluripotent stem cells that continuously differentiate to all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. Here, by combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from the transcriptional changes, and a novel lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterize gene expression changes during differentiation and discover cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at unprecedented resolution.

Attached files

Authors

Plass, Mireya
Solana, Jordi
Wolf , F. Alexander
Ayoub, Salah
Misios, Aristotelis
Gla┼żar, Petar
Obermayer, Benedikt
Theis, Fabian J.
Kocks, Christine
Rajewsky, Nikolaus

Oxford Brookes departments

Faculty of Health and Life Sciences\Department of Biological and Medical Sciences

Dates

Year of publication: 2018
Date of RADAR deposit: 2018-07-06


Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License


Related resources

This RADAR resource is the Accepted Manuscript of Cell type atlas and lineage tree reconstruction of whole adult animals by single-cell transcriptomics

Details

  • Owner: Joseph Ripp
  • Collection: Outputs
  • Version: 1 (show all)
  • Status: Live